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1. Non-chromatographic Purification of Synthetic DNA |
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Crude synthetic DNA contains desired full-length sequences mixed with failure strands. To separate them, the successful sequences are selectively attached during syntheses to a polymerizable group through a cleavable linker. After synthesis, the crude DNA is subjected to polymerization in the presence of monomers. Only the desired full-length sequences are attached to the resulting polymer; failure strands and other impurities are removed by washing. The full-length sequences are then cleaved from the polymer and pure DNA is obtained by extraction. Organic Letters 2010, 12, 3720-3723. |
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In another method, the undesired truncated failure sequences are capped during automated oligonucleotide synthesis with a polymerizable group. The desired full-length sequence is not. After synthesis, the failure sequences are polymerized and removed by filtration. Pure full-length DNA is obtained by evaporation of the mother liquor and water extracts. Chemical Communications 2011, 47, 1345-1347. |
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3. Cycloisomerization of Alkynyl Epoxides and Alcohols |
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4. More Sustainable Catalyst for Enyne Isomerization |
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5. Simple Method for Mono-acylation of Symmetric Diamines |
